Microbes aren’t known for their artistic merit, but that hasn’t stopped scientists from using bacteria to find their inner Ansel Adams. Bioengineer Chris Voigt and his team at the Massachusetts Institute of Technology have hacked a harmless strain of Escherichia coli so that it produces black pigment in darkness or, in red light, remains transparent. The result: an organism that behaves like film.
In their basic setup, red light shines through a printed transparency that’s taped to the bottom of an agar-filled petri dish. Only bacteria growing in the transparency’s shadows produce pigment. Voigt and his team borrowed two proteins from blue green algae to give E. coli this new ability. The proteins detect red light and turn off a gene that makes black pigment.
Jeff Tabor, a bioengineer at Rice University who collaborates with Voigt, is now engineering light-sensitive strains of E. coli to produce red and green pigments. If anyone can coax these bacteria to produce blue pigment, he says, the world could see its first bacterial pixel—the foundation of microbial television screens.
Time: 1 to 3 days
Cost: About $50 for six bacterial photos
Difficulty: 4 out of 5
Most companies ship biotech supplies only to registered laboratories. Fortunately, most DIY and community biology labs qualify.
- 30 μL of Voigt’s photographic bacteria
NOTE: You need to submit a request to order both the bacteria and the DNA to transform them into light-reactive organisms. Start by filling out this questionnaire.
- Agar mixture
- 100 mL luria broth agar
- 30 mg S-gal
- 50 mg iron ammonium sulfate
- 1 g low-melting-point agarose
- Antibiotics (not necessary, but they help prevent contamination):
- kanamycin (50 milligrams per milliliter of water)
- ampicillin (50 milligrams per milliliter of water)
- chloramphenicol (34 milligrams per milliliter of ethanol)
- Microwave oven
- Two microwave-safe containers with lids:
- One to hold 100 milliliters of agar One to hold 15 milliliters of agar
- Six 9-cm-diameter petri dishes
- 9-cm-diameter designs printed onto transparencies (sized to fit the round petri dishes)
- Plastic wrap
- Clear tape
- Red-light source
- Razor blade
1. Melt 15 milliliters of agar mixture in a microwave (about one minute on high)in the smaller container. Let cool until warm to the touch.
2. Add the bacteria and antibiotics (if you have them). Mix vigorously, but do not create bubbles.
3. Pour the mixture into a petri dish and quickly cover with a lid. Let it cool until solid, about 20 minutes.
4. Remove the lid, cover the petri dish with plastic wrap, and cut three narrow slits in the plastic.
5. Tape the transparency to the bottom of the petri dish, flip it over, and shine red light down onto the transparency for one to three days. (Make sure the light doesn’t melt the agar, as this will kill the bacteria.)
6. Admire your work. The petri-dish photograph can last for several years in a refrigerator.
How It Works
1. Proteins on the bacteria’s surface work together to detect red light. When light is present, the proteins prevent unique signaling molecules from forming within the cells.
2. These signaling molecules normally latch onto DNA inside the bacteria, then turn on a gene
that produces the enzyme beta-galactosidase.
3. The enzyme converts an agar additive called S-gal into dark pigment. Without signaling molecules, no pigment forms—so E. coli in darkness turn black and lit-up cells stay clear.
This article originally appeared in the September 2013 issue of Popular Science.
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